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10058 f4  (MedChemExpress)


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    MedChemExpress 10058 f4
    10058 F4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10058+f4/pm42032729-43-5-9?v=MedChemExpress
    Average 96 stars, based on 76 article reviews
    10058 f4 - by Bioz Stars, 2026-07
    96/100 stars

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    (A) In vitro fibrillation assays using recombinant c-MYC proteins (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (B) ELISA quantitation of A11 + –AOs generated by recombinant c-MYC proteins in vitro (mean ± SD, n=3 independent experiments, One-way ANOVA). (C) TEM visualization of protofibrils formed by recombinant c-MYC proteins in vitro (representative images of three independent experiments). Scale bars: 100 nm. (D) In vitro fibrillation assays using recombinant c-MYC/MAX dimers (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (E) TEM visualization of recombinant c-MYC/MAX dimers in vitro (representative images of three independent experiments). Scale bars: 100 nm. (F) Quantitation of endogenous c-MYC AOs in HeLa cells treated <t>with</t> <t>10058-F4</t> by In-Cell PLA ELISA (mean ± SD, n=3 independent experiments, One-way ANOVA).
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    (A) In vitro fibrillation assays using recombinant c-MYC proteins (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (B) ELISA quantitation of A11 + –AOs generated by recombinant c-MYC proteins in vitro (mean ± SD, n=3 independent experiments, One-way ANOVA). (C) TEM visualization of protofibrils formed by recombinant c-MYC proteins in vitro (representative images of three independent experiments). Scale bars: 100 nm. (D) In vitro fibrillation assays using recombinant c-MYC/MAX dimers (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (E) TEM visualization of recombinant c-MYC/MAX dimers in vitro (representative images of three independent experiments). Scale bars: 100 nm. (F) Quantitation of endogenous c-MYC AOs in HeLa cells treated <t>with</t> <t>10058-F4</t> by In-Cell PLA ELISA (mean ± SD, n=3 independent experiments, One-way ANOVA).
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    10058 f4  (Thermo Fisher)
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    (A) In vitro fibrillation assays using recombinant c-MYC proteins (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (B) ELISA quantitation of A11 + –AOs generated by recombinant c-MYC proteins in vitro (mean ± SD, n=3 independent experiments, One-way ANOVA). (C) TEM visualization of protofibrils formed by recombinant c-MYC proteins in vitro (representative images of three independent experiments). Scale bars: 100 nm. (D) In vitro fibrillation assays using recombinant c-MYC/MAX dimers (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (E) TEM visualization of recombinant c-MYC/MAX dimers in vitro (representative images of three independent experiments). Scale bars: 100 nm. (F) Quantitation of endogenous c-MYC AOs in HeLa cells treated <t>with</t> <t>10058-F4</t> by In-Cell PLA ELISA (mean ± SD, n=3 independent experiments, One-way ANOVA).
    10058 F4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    Image Search Results


    (A) In vitro fibrillation assays using recombinant c-MYC proteins (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (B) ELISA quantitation of A11 + –AOs generated by recombinant c-MYC proteins in vitro (mean ± SD, n=3 independent experiments, One-way ANOVA). (C) TEM visualization of protofibrils formed by recombinant c-MYC proteins in vitro (representative images of three independent experiments). Scale bars: 100 nm. (D) In vitro fibrillation assays using recombinant c-MYC/MAX dimers (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (E) TEM visualization of recombinant c-MYC/MAX dimers in vitro (representative images of three independent experiments). Scale bars: 100 nm. (F) Quantitation of endogenous c-MYC AOs in HeLa cells treated with 10058-F4 by In-Cell PLA ELISA (mean ± SD, n=3 independent experiments, One-way ANOVA).

    Journal: bioRxiv

    Article Title: c-MYC is an aggregation-prone, amyloidogenic protein

    doi: 10.64898/2026.03.12.711438

    Figure Lengend Snippet: (A) In vitro fibrillation assays using recombinant c-MYC proteins (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (B) ELISA quantitation of A11 + –AOs generated by recombinant c-MYC proteins in vitro (mean ± SD, n=3 independent experiments, One-way ANOVA). (C) TEM visualization of protofibrils formed by recombinant c-MYC proteins in vitro (representative images of three independent experiments). Scale bars: 100 nm. (D) In vitro fibrillation assays using recombinant c-MYC/MAX dimers (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (E) TEM visualization of recombinant c-MYC/MAX dimers in vitro (representative images of three independent experiments). Scale bars: 100 nm. (F) Quantitation of endogenous c-MYC AOs in HeLa cells treated with 10058-F4 by In-Cell PLA ELISA (mean ± SD, n=3 independent experiments, One-way ANOVA).

    Article Snippet: All chemicals were purchased commercially, including Thioflavin T (ThT) (cat# AC211760050, ThermoFisher Scientific Inc.), Congo red (CR) (cat#C580-25, ThermoFisher Scientific Inc.), Janus Green B (cat# AC191680050, Fisher Scientific LLC), and 10058-F4 (cat#T3048, TargetMol Chemicals Inc.). pcDNA-GFP and pcDNA3-2xHA-MYC were gifts from Martine Roussel (Addgene plasmid#74165 and 74164, respectively). pcDNA3-2xHA-MYC ΔC , 2xHA-MYC ΔC+ΔP2 , 2xHA-MYC ΔC+ΔP , and 2xHA-MYC ΔC+ΔP2+ΔP were generated using the Q5 Site-Directed Mutagenesis kit (cat#E0554S, New England Biolabs Inc.). pMYC-SEAP was purchased from Clontech Laboratories (Cell signaling pathway profiling systems, cat#631910) and pCMV-Gaussia Luc was purchased from Thermo Fisher Scientific (cat#16147).

    Techniques: In Vitro, Recombinant, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Generated

    (A) In vitro fibrillation assays using recombinant c-MYC proteins (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (B) ELISA quantitation of A11 + –AOs generated by recombinant c-MYC proteins in vitro (mean ± SD, n=3 independent experiments, One-way ANOVA). (C) TEM visualization of protofibrils formed by recombinant c-MYC proteins in vitro (representative images of three independent experiments). Scale bars: 100 nm. (D) In vitro fibrillation assays using recombinant c-MYC/MAX dimers (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (E) TEM visualization of recombinant c-MYC/MAX dimers in vitro (representative images of three independent experiments). Scale bars: 100 nm. (F) Quantitation of endogenous c-MYC AOs in HeLa cells treated with 10058-F4 by In-Cell PLA ELISA (mean ± SD, n=3 independent experiments, One-way ANOVA).

    Journal: bioRxiv

    Article Title: c-MYC is an aggregation-prone, amyloidogenic protein

    doi: 10.64898/2026.03.12.711438

    Figure Lengend Snippet: (A) In vitro fibrillation assays using recombinant c-MYC proteins (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (B) ELISA quantitation of A11 + –AOs generated by recombinant c-MYC proteins in vitro (mean ± SD, n=3 independent experiments, One-way ANOVA). (C) TEM visualization of protofibrils formed by recombinant c-MYC proteins in vitro (representative images of three independent experiments). Scale bars: 100 nm. (D) In vitro fibrillation assays using recombinant c-MYC/MAX dimers (mean ± SEM, n=3 independent experiments, Two-way ANOVA). (E) TEM visualization of recombinant c-MYC/MAX dimers in vitro (representative images of three independent experiments). Scale bars: 100 nm. (F) Quantitation of endogenous c-MYC AOs in HeLa cells treated with 10058-F4 by In-Cell PLA ELISA (mean ± SD, n=3 independent experiments, One-way ANOVA).

    Article Snippet: All chemicals were purchased commercially, including Thioflavin T (ThT) (cat# AC211760050, ThermoFisher Scientific Inc.), Congo red (CR) (cat#C580-25, ThermoFisher Scientific Inc.), Janus Green B (cat# AC191680050, Fisher Scientific LLC), and 10058-F4 (cat#T3048, TargetMol Chemicals Inc.). pcDNA-GFP and pcDNA3-2xHA-MYC were gifts from Martine Roussel (Addgene plasmid#74165 and 74164, respectively). pcDNA3-2xHA-MYC ΔC , 2xHA-MYC ΔC+ΔP2 , 2xHA-MYC ΔC+ΔP , and 2xHA-MYC ΔC+ΔP2+ΔP were generated using the Q5 Site-Directed Mutagenesis kit (cat#E0554S, New England Biolabs Inc.). pMYC-SEAP was purchased from Clontech Laboratories (Cell signaling pathway profiling systems, cat#631910) and pCMV-Gaussia Luc was purchased from Thermo Fisher Scientific (cat#16147).

    Techniques: In Vitro, Recombinant, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Generated